Waters Corporation has recently introduced its newest member of ACQUITY systems, the water ACQUITY UPSFC system, a holistically designed analytical system combining the advantages of sub -2um particle technology and the efficiency of supercritical fluid chromatograph (SFC).Built on proven Waters UltraPerformance Liquid Chromatography (UPLC). Technology, this new system offers up to 10x shorter run times, reduces solvent usage by as much as 95 per cent and cuts the cost of analytical by as much as 99 per cent; while giving laboratories a greener alternative to normal phase chromatography for chiral and achiral Separations.
The ACQUITY UPSFC System enables scientists to conduct normal phase chromatographic separations using carbon dioxide (CO2) as the primary mobile phase, rather than expensive and toxic solvents, saving laboratories tens of thousands of dollars over the lifetime of an instrument. The new UPSFC System offers laboratories significant advantages over traditional HPLC for routine UPSFC separations in research or regulated laboratories across many industries
In 2004, Waters revolutionized LC with the introduction of sub-two micron UPLC separations resulting in significantly better data quality. Today this proven and reliable technology offers that same potential for analytical SFC,'' said Harbaksh sidhu, General Manager, Waters SFC Operations. ' The UPSFC System provides the same impressive separation, resolution and speed attributes of UPLC with the outstanding green benefits of SFC,'' he further remarked. Using specially designed Viridis columns, the UPSFC System offers the ruggedness and dependability scientists are looking for, with the ease of use they've come to expect from LC. The System is fully compatible with Waters industry-leading Empower and MassLynx informatics software and Waters line of UV (ultraviolet) and mass detectors.
Supercritical fluids share the physical properties of both a gas and a liquid and exhibit the positive chromatographic qualities of both. Relative to HPLC solvents, their low viscosity and high solute diffusivity result in faster analysis times, lower back-pressure, shorter re-equilibration times and higher throughput without loss in efficiency. For chiral and achiral separations, this means greater signal-to noise ratios, better peak shapes and higher peak capacity enabling scientists to rapidly confirm the purity of compounds down to the 0.01 per cent level, determine the enantiomeric excess, of when coupled to mass spectrometry, confirm compound identity.
In the 1990s, as the number of chiral drugs exceeded the number of achiral drugs, the FDA published regulatory guidance regarding enantiomeric drug compounds. SFC quickly caught on with scientists involved in drug discovery and found its niche in purification of chiral compounds. With advancements in instrument and column technology, and a focus on sustainability, the advantages of SFC have not gone unnoticed by other scientists and SFC is experiencing a resurgence in applications areas not limited to just purification but also analysis including: fossil fuels & hydrocarbons, agrichemicals, polymers, explosives & products, industrial chemicals, metal chelates and organometallic compounds.
UPSFC technology consumes a fraction of the solvent of traditional HPLC systems. And compared to the cost of solvents like acetonitrile, commercial grade CO2 is very inexpensive Furthermore; CO2 costs nothing to dispose of, naturally reverting to its gaseous state. Inert and nonflammable, CO2 is non-toxic and safer for laboratory personnel to handle. For laboratories seeking to cut costs and putting the principles of green chemistry into action, ACQUITY UPSFC System offers impressive advantages and is an ideal choice as an alternative to normal phase chromatography.